#Minitube boar semen freezing plus#

Sixty-nine Landrace multiparous sows were randomly allocated into two groups, including control (n=36) and treatment (n=33). The aim of the present study was to determine the conception rate and litter size in sows after fixed time intra-uterine insemination using frozen-thawed boar semen in a commercial swine herd in Thailand. It could be concluded that both types of extender used during the holding time and the thawing medium significantly influenced FT boar sperm quality. Thawing of cryopreserved boar semen in BTS yielded a significantly higher subjective motility than thawing in seminal plasma (20.3% and 16.9%, p= 0.021). It was found that semen extended in extender D for 2 hours before cryopreservation yielded a higher individual motility than those extended in extender B (20.9% and 16.9%, p= 0.045) and tended to be higher than those extended in extender A (17.5%, p= 0.090).

The post-thawed sperm qualities including subjective motility, sperm viability, functional integrity of the sperm plasma membrane and acrosome integrity were evaluated. The semen was divided into two groups: the control group was diluted in BTS the treatment group was diluted in seminal plasma. Thawing was achieved by immersing the straws in 50 0 C water for 12 sec. The semen were loaded into 0.5 ml straws and frozen.

#Minitube boar semen freezing plus#

The sperm pellets were diluted in lactose-egg yolk extender plus glycerol and Equex STM Paste. The extended semen was cooled down and centrifuged. Sperm-rich fractions were collected from 11 boars and were divided into four groups according to the type of semen extender used during the holding time (A, B, C and D). The present study investigated the influence of four different commercial semen extenders used during the holding time and the use of seminal plasma as a thawing medium on the frozen-thawed (FT) boar semen quality. The results of these investigations suggested that Equex-STM exerts a beneficial effect on the quality of cryopreserved boar semen and this cryopreservation protocol was favourable for a 0.5-ml straw. Results showed that the individual motility, viability and acrosomal normal apical ridge (NAR) were improved (p 0.05). Thawing was achieved in warm water at 50 degrees C for 12 s and then was incubated in a 38 degrees C water-bath for 30 min before evaluating sperm quality. The straws were placed in liquid nitrogen (LN(2)) vapour approximately 3 cm above the level of LN(2) for 20 min and then were plunged into LN(2). The extended semen was loaded into either 0.25- or 0.5-ml straws. Three ejaculates from each of four purebred boars (three Landrace and one Yorkshire) were collected and frozen with a lactose-egg yolk extender containing glycerol with or without 1.5% Equex-STM.

The present experiments were designed to study the effect of adding the detergent Equex-STM to freezing extender, and of straw volume (0.25 ml vs 0.5 ml), on boar sperm quality after cryopreservation.